By Linda on Oct 14, 2010 in Infections | comments(0)
Full article: http://www.ncbi.nlm.nih.gov/pubmed/19772602
Excerpt:
BACKGROUND: Contamination of vertebrate cell lines with animal retroviruses has been documented repeatedly before. Although such viral contaminants can be easily identified with high sensitivity by PCR, it is impossible to screen for all potential contaminants. Therefore, we explored two novel methods to identify viral contaminations in cell lines without prior knowledge of the kind of contaminant. RESULTS: The first hint for the presence of contaminating retroviruses in one of our cell lines was obtained by electron microscopy of exosome-like vesicles released from the supernatants of transfected 293T cells. Random amplification of particle associated RNAs (PAN-PCR) from supernatant of contaminated 293T cells and sequencing of the amplicons revealed several nucleotide sequences showing highest similarity to either murine leukemia virus (MuLV) or squirrel monkey retrovirus (SMRV). Subsequent mass spectrometry analysis confirmed our findings, since we could identify several peptide sequences originating from monkey and murine retroviral proteins. Quantitative PCRs were established for both viruses to test currently cultured cell lines as well as liquid nitrogen frozen cell stocks. Gene fragments for both viruses could be detected in a broad range of permissive cell lines from multiple species. Furthermore, experimental infections of cells negative for these viruses showed that both viruses replicate rapidly to high loads. We decided to further analyze the genomic sequence of the MuLV-like contaminant virus.
By Linda on Jul 21, 2010 in Infections | comments(1)
Linda’s comment: I have used tea tree oil on toe nails for fungus, but wouldn’t think about it for dissolving biofilms. I have been successfully been dissolving biofilms with the FIGHT protocol. People ask me how I can tell. (For those who have weak stomachs stop reading here)….You can find them splattered on the sides of your toilet, like spider webs….it takes a strong brush to wash them away. I also take at least 2 Boluoke capsules a day, and at least 8 to 10 Wobenzyme 3 times daily on empty stomach. I did use a loading dose of one Boluoke 3 times daily and took the 10 Wobenzyme 4 to 5 times daily for the first 90 days. At that time is when I added a teaspoon of the ZeoGold along with my ACZnano Zeolite.
Biofilms are not just Lyme disease, but other diseases, but a good daily detox is a MUST if you have Lyme disease. Reducing your total body burden of pathogens and toxins is vital for getting control of Lyme disease. We have a lot to deal with today with the Gulf Oil spill, which BTW, is effecting our health and is going to get worse, but we are having to fight the GMO foods and exposures to pesticides and herbicides. With a good daily detox we can help to neutralize the hits we are taking daily from our environmental toxins.
Go to the Webinar’s I have posted on this blog and learn about the FIGHT protocol. You will also learn what you need to do to FIGHT these problems to help protect your health.
Excerpt:
Objective: This study aimed to investigate antimicrobial treatment of an infected cochlear implant, undertaken in an attempt to salvage the infected device.
Methods: We used the broth microdilution method to assess the susceptibility of meticillin-sensitive Staphylococcus aureus isolate, cultured from an infected cochlear implant, to common antimicrobial agents as well as to novel agents such as tea tree oil. To better simulate in vivo conditions, where bacteria grow as microcolonies encased in glycocalyx, the bactericidal activity of selected antimicrobial agents against the isolate growing in biofilm were also compared.
Results: When grown planktonically, the S aureus isolate was susceptible to 17 of the 18 antimicrobials tested. However, when grown in biofilm, it was resistant to all conventional antimicrobials. In contrast, 5 per cent tea tree oil completely eradicated the biofilm following exposure for 1 hour.
By Linda on Nov 16, 2009 in Linda's Tips | comments(4)
Linda’s Comment: I’m in the process of reading this book and so far it ROCKS. Having battled (5) Cancers myself and beaten them on using ONLY alternative medicine and adjuncts, I can relate to much of what Suzanne has to say. Cancer is preventable, but many of us don’t pay attention until we hear those words,”You have Cancer”….Yes, it certainly gets your attention. Many forget to stop and think about how they ended up with Cancer? These are things you don’t think of ,UNTIL you hear those words, and then it is usually the last thing you even think about. Once I got over the shock of hearing those words and had my treatment protocol laid out, I wanted to know how and why? No, I didn’t question God, but I questioned myself. Thanks to my alternative doctors and friends I stepped back and began looking at what I ate. Yep, I ate a lot of GMO foods, sugar, the wrong fats and used a microwave. I began looking under my cabinets and in my medicine cabinets, as well as my laundry room. Boy was I shocked. My friends, doctors and research lead me to start removing all the toxins and chemicals I had around me. It actually isn’t hard to do, but it is a shocker that the FDA would allow the industry to sells these DEADLY products.
The chemical toxins that we use to clean and wash our clothes with are scary when you begin to read labels. The chemicals we put on our bodies and the cosmetics that are toxic and loaded with chemicals will make you angry, as they did me. How could the industry approve them? How could the industry in good faith shove these chemicals at us? I had no idea what perfume and body creams were putting into my blood stream, much less in my lungs, breasts and ovaries!! The plug-in air-fresheners and aerosol sprays are DEADLY. The good news is there are cleaning products, laundry products, body creams, shampoos that are not toxic and filling our bodies with additional toxins. Oh yea, let’s not forget the pesticides and herbicides that are giving us our toxic world!!
I only wish I had known about the FIGHT program, that I began over a year ago. Thank you Dr Gordon for developing this lifelong daily detox protocol. IT WORKS….They talk about the FIGHT program in the 16th chapter of Suzanne’s book. The FIGHT protocol has helped me begin to dissolve biofilms and reduce my total body burden of pathogens and toxins. The great thing about this program is it is lifelong. Yes, I said lifelong, but we are living in a very toxic world and are being slammed daily with hundreds of toxins from our environment. Continued
By Linda on Jul 31, 2009 in Infections, Toxins | comments(12)
Summation: Biofilms of Borrelia Burgdorferi by Alan B. MacDonald, MD, May 17, 2008
1) Biofilms of Borrelia are indispensable elements for species survival in hostile environments.
2) Biofilms of borrelia provide protection to the microbes which live inside of the matrix.
3) DNA of Borrelia (externalized) constitutes a portion of the borrelia biofilm matrix.
4) Exchange of genomic material occurs between the borrelia in the biofilm.
5) Morphologic diversity of borrelia within biofilms (cyst, granular, L form, and spiral forms) is
evident.
Biofilms has become one of the hottest topics within the Lyme communities. Doctors from all parts of the world are focusing on biofilms and how it effects treatments of chronically ill patients, as well as Lyme patients. Pathogens and toxins we are carrying in our bodies prevent us from beginning the dissolving process. We must begin a process to help dissolve these pathogens and toxins that are helping to keep the biofilms in place.
I have been using several things to break down the biofilms, which are residing in my body.
Continued
