By Linda on May 16, 2011 in Infections | comments(0)
Linda’s comment: in vitro model of the human BBB mimics many of the important features of in vivo B. burgdorferi interactions with the BBB. Hence, this model should be an important tool for identifying the cellular and molecular elements implicated in B. burgdorferi interactions with the BMEC, as well as for helping characterize the biochemical mechanisms by which the bacteria cross the BBB.
Link: http://iai.asm.org/cgi/content/full/73/2/1014
Excerpt:
Our in vitro model of the human BBB mimics many of the important features of in vivo B. burgdorferi interactions with the BBB. Hence, this model should be an important tool for identifying the cellular and molecular elements implicated in B. burgdorferi interactions with the BMEC, as well as for helping characterize the biochemical mechanisms by which the bacteria cross the BBB.
By Linda on Aug 27, 2010 in F.I.G.H.T., Food, Toxins | comments(0)
Excerpt:
Blood-brain barrier (BBB) disruption occurs during human immunodeficiency virus encephalopathy, but the mechanisms involved are not understood. We studied how acute and ongoing exposure to human immunodeficiency virus 1 envelope gp120 alters BBB structure and permeability. Intravenous Evans blue, given before stereotaxic gp120 injection into the caudate putamen of rats, was rapidly extravasated. Gelatinolytic activity, studied by in situ zymography, was increased after gp120 administration and was localized within cerebralvessel walls. The gp120 increased the expression of matrix metalloproteinases (MMPs) 2 and 9. Laminin and claudin-5, key BBB components and targets of both MMPs, were greatly reduced upon gp120 administration. The gp120 increased lipid peroxidation in the vascular endothelium and in neurons. Prior administration of rSV40 vectors carrying the antioxidant enzymes Cu/Zn superoxide dismutase or glutathione peroxidase protected from gp120-induced BBB damage. N-methyl-D-aspartate receptor activation upregulated pro-MMP-9 and increased MMP-9 gelatinase activity, and memantine, an N-methyl-D-aspartate receptor blocker, mitigated gp120-induced BBB abnormalities. Using intra-caudate putamen SV(gp120) to test the effects of chronic exposure to expressed gp120, we determined that oxidant stress and increased BBB permeability occurred as in acute exposure. These data indicate that both direct administration and cellular expression of gp120 lead to disruption of the BBB by increasing MMPs and reducing vascular tight junction proteins via mechanisms involving reactive oxygen species generation and oxidant injury.
By Linda on Jul 13, 2010 in Toxins | comments(0)
Linda’s Comments….here is another headsUP on the effects of mercury and our immune system. Adding one more neurotoxin to our body when it isn’t necessary is not a safe way of playing the game of roulette with toxins and pathogens. Those of us with chronic illness are continuously fighting this toxin game…..You NEED to begin a journey of detox folks…..Just using zeolite is a beginning BUT when releasing this neurotoxin make sure you are taking a good binder…I personally like the www.longevityplus.com Beyond Fiber….One of the best fibers I have found….It doesn’t just pull the bad things from your colon but it can replenish the good that is removed when using detox products.
Excerpt:
Methods
Human leukemic cultured LAD2 mast cells and normal human umbilical cord blood-derived cultured mast cells (hCBMCs) were stimulated by HgCl2 (0.1-10 microM) for either 10 min for beta-hexosaminidase release or 24 h for measuring vascular endothelial growth factor (VEGF) and IL-6 release by ELISA.
Results
HgCl2 induced a 2-fold increase in beta-hexosaminidase release, and also significant VEGF release at 0.1 and 1 microM (311+/-32 pg/10*6 cells and 443+/-143 pg/10*6 cells, respectively) from LAD2 mast cells compared to control cells (227+/-17 pg/10*6 cells, n=5, p<0.05). Addition of HgCl2 (0.1 microM) to the proinflammatory neuropeptide substance P (SP, 0.1 microM) had synergestic action in inducing VEGF from LAD2 mast cells. HgCl2 also stimulated significant VEGF release (360 +/- 100 pg/10*6 cells at 1 microM, n=5, p<0.05) from hCBMCs compared to control cells (182 +/-57 pg/10*6 cells), and IL-6 release (466+/-57 pg/10*6 cells at 0.1 microM) compared to untreated cells (13+/-25 pg/10*6 cells, n=5, p<0.05). Addition of HgCl2 (0.1 microM) to SP (5 microM) further increased IL-6 release.
Conclusions
HgCl2 stimulates VEGF and IL-6 release from human mast cells. This phenomenon could disrupt the blood-brain-barrier and permit brain inflammation. As a result, the findings of the present study provide a biological mechanism for how low levels of mercury may contribute to ASD pathogenesis.
By Linda on Apr 22, 2010 in F.I.G.H.T. | comments(0)
Linda’s comments:..This is a very important subject for all folks, especially those with chronic illness. It is very hard to get well when you are being blasted by EMF/EMR. One of the things I have noticed is the more I detox the more sensitive I am to EMF/EMR. I’m GRATEFUL for this, as it is a sure sign that I am being blasted more than what I ever imagined.
I am being treated by a new technology called Energy Enhancement System and you can read more at www.eesystem.com ……These treatments are helping me to eliminate the EMF/EMR’s from my body. The exciting thing about these treatments is I take my lifelong daily detox protocol FIGHT before I enter the room for a 2 hour session….the system ENHANCES the protects I am taking for the FIGHT program.
There are many who are buying these systems for their homes….I wish I could afford a system.
Please read all the information below regarding EMF/EMR and learn. When a chronically ill person is getting too much EMF/EMR they can will not reach the wellness level they want to reach.
Linda
Excerpt:
A report that cited more than 2,000 studies found that chronic exposure to even low-level radiation (like that from cell phones) can cause a variety of cancers, impair immunity, and contribute to Alzheimer’s disease and
dementia, heart disease, and many other ailments. One likely way: EMFs open the blood-brain barrier, causing blood vessels to leak fluid into the brain and damage neurons.
What’s more, a less–well known kind of EMF, known as “dirty” or transient electricity #, may play an even more damaging role. Transients are largely by-products of modern energy-efficient electronics and appliances—from computers, refrigerators, and plasma TVs to compact fluorescent light bulbs and dimmer switches—which tamp down the electricity they use. This manipulation of current creates a wildly fluctuating and potentially dangerous electromagnetic field that essentially charges up the electrons in every cell of your body. Some research suggests that by overlapping the body’s signaling mechanisms, transients may interfere with the secretion of insulin, drown out the call and response of the immune system, and cause other physical havoc.
