All Posts Tagged With: "ELISA"

Link: http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=21117995&retmode=ref&cmd=prlinks

Excerpt:

Results-Sensitivity and specificity of the in-clinic ELISA for detection of
heartworm antigen (99.2% and 100%, respectively), antibodies against B
burgdorferi (98.8% and 100%, respectively), and antibodies against E canis
(96.2% and 100%, respectively) were similar to results for a similar
commercial ELISA. In samples obtained from dogs in the northeast and upper
Midwest of the United States, sensitivity and specificity of the in-clinic
ELISA for antibodies against Anaplasma spp were 99.1% and 100%,
respectively, compared with results for an immunofluorescence assay. Samples
from 2 dogs experimentally infected with the NY18 strain of A
phagocytophilum were tested by use of the in-clinic ELISA, and antibodies
against A phagocytophilum were detected by 8 days after inoculation.
Antibodies against Anaplasma platys in experimentally infected dogs
cross-reacted with the A phagocytophilum analyte. Coinfections were
identified in several of the canine serum samples. 
Conclusions and Clinical Relevance-
The commercially available in-clinic ELISA could be used by veterinarians to
screen dogs for heartworm infection and for exposure to tick-borne
pathogens.

Link: http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?bfrom=pubmed&id=21271270&retmode=ref&cmd=prlinks

Excerpt:

We investigated the influence of assay choice on the results in a two-tier
testing algorithm for the detection of anti-Borrelia antibodies.

Eighty-nine serum samples from clinically well-defined patients were tested in eight
different enzyme-linked immunosorbent assay (ELISA) systems based on whole-cell
antigens, whole-cell antigens supplemented with VlsE and assays using
exclusively recombinant proteins.

A subset of samples was tested in five immunoblots: one whole-cell blot, one
whole-cell blot supplemented with VlsE and three recombinant blots.

The number of IgM- and/or IgG-positive ELISA results in
the group of patients suspected of Borrelia infection ranged from 34 to 59%.

The percentage of positives in cross-reactivity controls ranged from 0 to 38%.

Comparison of immunoblots yielded large differences in inter-test agreement and
showed, at best, a moderate agreement between tests.

***Remarkably, some immunoblots gave positive results in samples that had been
tested negative by all eight ELISAs.***

The percentage of positive blots following a positive ELISA
result depended heavily on the choice of ELISA-immunoblot combination.

We conclude that the assays used to detect anti-Borrelia antibodies have widely
divergent sensitivity and specificity.

Full article: http://www.prohealth.com/library/showarticle.cfm?libid=15636 

Excerpt:

Results: In a total of 125 patients, there were no significant differences in clinical presentation by sex.

The initial self-misdiagnosis rates for men and women were 10% and 18%, respectively (P = NS).

Among the 62 patients with a serologic test as part of their clinical evaluation:

• 50% of men had a positive, 2-tier result

• Compared with 32% of women (P = NS).

Among the 41 patients with a positive ELISA, median ELISA values (3.4 vs 2.0; P = 0.03) and median number of immunoglobulin G (IgG) bands (4 vs 2; P = 0.03) were significantly higher among men.

Conclusions: In this small, retrospective sample, we found evidence for sex-based differences in the magnitude of ELISA and IgG serologic response to early Lyme disease. 

Such differences could have implications for appropriate diagnosis, treatment, and disease classification.

Larger, prospective studies are needed to replicate the results found in this study and to examine their relationship to sex-based immunologic variability.

Source: Gender Medicine, Aug 2010;7(4):320-9. PMID: 20869632, by Schwarzwalder A, Schneider MF, Lydecker A, Aucott JN.The Lyme Disease Research Foundation of Maryland, Lutherville, Maryland, USA.

Full article: http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=20734722&retmode=ref&cmd=prlinks

Excerpt:

RESULTS: Performed comparative clinico-laboratory analysis
demonstrated increased levels of LL-2, IL-4, and IL-8 in patients
during acute phase of tick-borne borreliosis that could point to
host’s response on bacterial infection. It should be noted that
in patients with arthritis levels of LL-4 and
IL-2 remained high during recovery phase that probably determined
by possible persistence of Borrelia burgdorferi. 
CONCLUSION: Further research of cytokines during Lyme borreliosis
could have important diagnostic and prognostic value.

Full article: http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=20734722&retmode=ref&cmd=prlinks

Excerpt:

RESULTS: Performed comparative clinico-laboratory analysis
demonstrated increased levels of LL-2, IL-4, and IL-8 in patients
during acute phase of tick-borne borreliosis that could point to
host’s response on bacterial infection. It should be noted that
in patients with arthritis levels of LL-4 and
IL-2 remained high during recovery phase that probably determined
by possible persistence of Borrelia burgdorferi. 
CONCLUSION: Further research of cytokines during Lyme borreliosis
could have important diagnostic and prognostic value.

Full article: http://content.nejm.org/cgi/content/full/339/3/160

Excerpt:

Background Babesiosis, a zoonosis caused by the protozoan Babesia microti, is usually not treated when the symptoms are mild, because the parasitemia appears to be transient. However, the microscopical methods used to diagnose this infection are insensitive, and few infected people have been followed longitudinally. We compared the duration of parasitemia in people who had received specific antibabesial therapy with that in silently infected people who had not received specific therapy.

Methods Forty-six babesia-infected subjects were identified from 1991 through 1996 in a prospective, community-based study designed to detect episodes of illness and of seroconversion among the residents of southeastern Connecticut and Block Island, Rhode Island. Subjects with acute babesial illness were monitored every 3 months for up to 27 months by means of thin blood smears, Bab. microti polymerase-chain-reaction assays, serologic tests, and questionnaires

Full article: http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=20534091&retmode=ref&cmd=prlinks

Excerpt:

In the present study, we report the occurrence of borreliosis in
patients from the Brazilian Amazonic region. Nineteen (7.2%) out
of 270 dermatological patients with different skin diseases (no
one with clinical Lyme disease), tested positive by ELISA for
Borrelia burgdorferi. Serum samples from 15 out of the 19
ELISA-positive patients were further evaluated by Western blot.
Presence of Borrelia burgdorferi specific IgG was confirmed in
eight (53.3%) out of the
15 patients. All eight patients with ELISA and Western blot
positive reactions were treated with doxycycline, according to
the Centers for Disease Control and Prevention guidelines. One of
them had clinical manifestations of colagenosis and was sent to
the Department of Internal Medicine for further investigation.
Data presented here suggested that borreliosis “lato sensu” is in
the Brazilian Amazon region.

Full article: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010817

Excerpt:

Chronic Fatigue Syndrome (CFS) studies from our laboratory and
others described decreased natural killer cell cytotoxicity
(NKCC) and elevated proportion of lymphocytes expressing the
activation marker, dipeptidyl peptidase IV (DPPIV) also known as
CD26. However, neither these assays nor other laboratory tests
are widely accepted for the diagnosis or prognosis of CFS. This
study sought to determine if NKCC or DPPIV/CD26 have diagnostic
accuracy for CFS.

Excerpt:

The laboratory diagnosis of early disseminated Lyme borreliosis
(LB) rests on IgM and IgG antibodies in serum. The purpose of
this study was to refine the statistical interpretation of IgM
and IgG by combining the diagnostic evidence provided by the two
immunoglobulins and exploiting the whole range of the
quantitative variation in test values. ELISA assays based on
purified flagella antigen were performed on sera from 815 healthy
Danish blood donors as negative controls and 117 consecutive
patients with confirmed neuroborreliosis (NB).

Full article: http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=20402677&retmode=ref&cmd=prlinks

Full article: http://content.karger.com/produktedb/produkte.asp?typ=fulltext&file=000209229

Excerpt:

Lyme serology was positive for both IgG and IgM (ELISA, Enzygnost
Borreliosis , Siemens, Dade Behring, Germany, and blot,
Euroline WB , Euroimmun, Germany). A punch biopsy of a papule showed a dermal perifollicular ( fig. 2 ) – and occasionnally perineural – infiltrate of lymphocytes and plasma cells, consistent with a borrelial infection. Borrelia burgdorferi DNA was amplified from fresh tissue obtained from a skin biopsy performed on a peripilar papule, using a specific real-time PCR according to Mäkinen et al. [1] (culture not performed). The erythema resolved after a 3-week doxycycline treatment whereas arthralgia and dysesthesia persisted.