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Full article: http://www.lymeneteurope.org/forum/viewtopic.php?f=7&t=1336#p9502

Excerpt:

Abstract
Using PCR in conjunction with pre-enrichment culture, we detected Bartonella henselae and B. vinsonii subspecies berkhoffii in the blood of 14 immunocompetent persons who had frequent animal contact and arthropod exposure.

Attempts to isolate Bartonella sp. from immunocompetent persons with serologic, pathologic, or molecular evidence of infection are often unsuccessful; several investigators have indicated that Bartonella isolation methods need to be improved (1–4). By combining PCR and pre-enrichment culture, we detected B. henselae and B. vinsonii subspecies berkhoffii infection in the blood of immunocompetent persons who had arthropod and occupational animal exposure

The Study

From November 2004 through June 2005, blood and serum samples from 42 persons were tested, and 14 completed a questionnaire, approved by the North Carolina State University Institutional Review Board. Age, sex, animal contact, history of bites, environment, outdoor activity, arthropod contact, travel, and medical history were surveyed. Bacterial isolation, PCR amplification, and cloning were performed by using previously described methods (5–7). Each blood sample was tested by PCR after direct DNA extraction, pre-enrichment culture for at least 7 days, and subculture onto a blood agar plate (Figure). An uninoculated, pre-enrichment culture was processed simultaneously as a control. Methods used for DNA extraction and conventional and real-time PCR targeting of the Bartonella 16S-23S intergenic spacer (ITS) region and heme-binding protein (Pap31) gene have been described (7,8). Conventional PCR amplicons were cloned with the pGEM-T Easy Vector System (Promega, Madison, WI, USA); sequencing was performed by Davis Sequencing, Inc. (Davis, CA, USA). Sequences were aligned and compared with GenBank sequences with AlignX software (Vector NTI Suite 6.0 (InforMax, Inc., Bethesda, MD, USA) (7,8). B. vinsonii subsp. berkhoffii, B. henselae, and B. quintana antibodies were determined by using a modification of a previously described immunofluorescence antibody assay (IFA) procedure (9

Study participants included 12 women and 2 men, ranging in age from 30 to 53 years; all of them reported occupational animal contact for >10 years (Table). Most had daily contact with cats (13 persons) and dogs (12 persons). All participants reported animal bites or scratches (primarily from cats) and arthropod exposure, including fleas, ticks, biting flies, mosquitoes, lice, mites, or chiggers. All participants reported intermittent or chronic clinical symptoms, including fatigue, arthralgia, myalgia, headache, memory loss, ataxia, and paresthesia (Table). Illness was most frequently mild to moderate in severity, with a waxing and waning course, and all but 2 persons could perform occupational activities. Of the 14 participants, 9 had been evaluated by a cardiologist, 8 each by an infectious disease physician or a neurologist, and 5 each by an internist or a rheumatologist. Eleven participants had received antimicrobial drugs.

MINNEAPOLIS (AP) _ Deer ticks are expanding their range in the Upper Midwest and southern Canada, new ticks are moving into the area and existing ticks are picking up new diseases, increasing the threat of illness to hikers tramping through the region’s woods. Continued

Clinical practice guidelines are increasing in number. Unfortunately,
when scientific evidence is uncertain, limited, or evolving, as is often
the case, conflict often arises between guideline committees and
practicing physicians, who bear the direct responsibility for the care of
individual patients. The 2006 Infectious Diseases Society of America
guidelines for Lyme disease, which have limited scientific support,
could, if implemented, limit the clinical discretion of treating physicians
and the treatment options available to patients

Introduction

Clinical practice guidelines are now ubiquitous throughout the
United States. The National Guidelines Clearing House, under the
category “diseases,” currently lists 2,126 separate guidelines on its
web site. Clinical guidelines are intended to assist physicians in
patient care by clearly communicating the results of the guideline
committees’ evaluation of available therapeutic options. However,
the processes by which individual guidelines are constructed may be
less clear, leading to disagreements between the issuing committee
and the physicians who treat patients-physicians who may well be
as experienced and knowledgeable as the guideline committee.

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Linda’s Comment:  It amazes me that in the following publications do we find any suggestions about reducing our total body burden of pathogens and toxins.  It is a MUST that Lymies begin to reduce their total body burden of pathogens and toxins in order to begin addressing Lyme, Lyme Arthritis, Arthritis, and other chronic illness we see with Lyme patients.  Some people choose antibiotics…..I personally never went near antibiotics.  My whole detox and healing program was using anti-microbials, alternative medicine, alternative modalities, NO GMO foods, NO sugars, NO fast foods, NO soda’s, NO caffeine, NO coffee and I ate and still do eat organic foods.  I also have used a PhotonGenie since 2001 and use it daily.  I like it better than Rife, as I don’t have to worry about settings, I just turn it on and go.  I even sleep in mine.  The critters we Lymies fight LOVE heavy metals and especially GMO foods.  
 
There are also some foods that you don’t want to eat if you are having symptoms of Arthritis, however, it is more important to get rid of the GMO, sugars, coffee and soda’s to reduce the inflammation and pain.  The great thing about the fight program is you are dissolving biofilms and reducing inflammation on a daily basis.  So much of our pain comes from inflammation. 
 
I of course use many more things with my lifelong daily detox protocol.  If you can get IV chelation and do weekly colonics you can move things along faster.  However, you can start the program one step at a time and move at your own pace.  This is one protocol that must be done as suggested to get the full benefit of wellness.  The first three months are your toughest, but after that it is a breeze.  Yes, ever so often you have a day or two like you did when you first started, but I tell folks, it is like peeling an onion.  As you reach a new level you will have a couple of days where you keep your bathroom close.  At the end of 60 to 90 days and you begin to feel your life coming back to you, you will be very pleased that you began this journey.  Feel free to ask questions and I will share my journey with you.  Just remember JUST SAY NO TO GMO!! Continued