Lyme in China – differing DNA?
By Linda on Jan 22, 2011 in Infections
Excerpt:
Abstract Thirty-two strains of Borrelia burgdorferi sensu lato were isolated
from Borrelia burgdorferi Sensu Lato collected from northeastern China from May to
June in 2004 and 2005. Restriction fragment length polymorphism (RFLP)
analysis and sequence analysis of 5S-23S rRNA intergenic spacer revealed
that 29 (90.6%) belonged to Borrelia garinii, demonstrating B, C, and a
unique pattern. The remaining three isolates (9.4%) were Borrelia afzelii
with pattern D. The phylogenetic analysis based on 5S-23S rRNA intergenic
spacer showed that B.
garinii and B. afzelii genospecies clustered into two separate lineages. B.
garinii strains were classified into three different branches: All the
strains with RFLP pattern C were in the same branch, strain VH10 with a
unique RFLP pattern clustered with strains VH9 and MDH2 with pattern B, and
the rest of the strains with pattern B constitute another branch. These
findings demonstrate the genetic diversity of B. burgdorferi sensu lato
isolates from northeastern China.
