All Posts Tagged With: "polymerase chain reaction (PCR)"

Linda’s comment:  Clinical, diagnostic and immunological characteristics of patients with possible neuroborreliosis without intrathecal Ig-synthesis against Borrelia antigen in the cerebrospinal fluid.

PCR did not reveal any Borrelia antigen in CSF. The diagnosis
and treatment of possible but not confirmed neuroborreliosis is a
clinical challenge. The clinical response to treatment may be the best
option in these cases.

From the article:

The occurrence of Toxoplasma gondii and Borrelia burgdorferi sensu lato in
Ixodes ricinus ticks from east Poland with the use of pcr.

Sroka J, Szymanska J, Wojcik-Fatla A.

Department of Occupational Biohazards, Institute of Agricultural Medicine,
Jaczewskiego 2, 20-090 Lublin, Poland. jack@galen.imw.lublin.pl

715 Ixodes ricinus ticks from 4 regions of Lublin macroregion (Piotrowice,
Polesie National Park, Dabrowa and Leczynsko-Wlodawskie Lakeland) were examined
for the presence of Borrelia burgdorferi sensu lato and Toxoplasma gondi, using
polymerase chain reaction (PCR) and nested-PCR methods. The clonal type of
samples tested positive for T. gondii was identified by amplification and
fragmenting with restrictions enzymes (RFLP-restriction fragment length
polymorphism). Positive results were confirmed by sequencing.

To read more: http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=20047269&retmode=ref&cmd=prlinks
PMID: 20047269

A fatal case of transfusion-transmitted babesiosis in the State of Delaware
Yong Zhao, Kenneth R. Love, Scott W. Hall, and Frank V. Beardell

Background: Most cases of human babesiosis in North America are caused
by Babesia microti, which is endemic in the northeastern and upper
midwestern United States. Although the disease is usually transmitted by
a tick bite, there has been an increase in the number of
transfusion-transmitted cases reported. We describe a fatal case of
transfusion-transmitted babesiosis in a nonendemic state, Delaware. Continued