By Linda on Feb 28, 2011 in Infections | comments(0)
Link: http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=21289156&retmode=ref&cmd=prlinks
Excerpt:
In this study we compared some common Bartonella culturing methodologies
using four diverse species causing human illnesses. Based on a review of the
literature, we focused on three major inconsistencies between protocols:
base media, cell co-culture and temperature. Our data showed that B. tamiae
demonstrated temperature-dependent growth limitations between common
culturing conditions only 2 degrees C apart. Additionally, growth of B.
quintana was significantly enhanced by the presence of mammalian cell
co-culture within mammalian culture conditions, however when the media was
modified to incorporate insect culture-based media, co-culturing with
mammalian cells was no longer needed. In this study, we were able to
overcome these temperature and cell dependent limitations and accommodate
all of the strains tested by combining mammalian culture-based media with
insect culture-based media.
By Linda on Jun 29, 2010 in Infections | comments(0)
Full article: http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=20519614&retmode=ref&cmd=prlinks
Excerpt:
To determine the role of Bartonella species as causes of acute
febrile illness in humans from Thailand, we used a novel strategy
of co-cultivation of blood with eukaryotic cells and subsequent
phylogenetic analysis of Bartonella-specific DNA products.
Bartonella species were identified in 14 blood clots from febrile
patients. Sequence analysis showed that more than one-half of the
genotypes identified in human patients were similar or identical
to homologous sequences identified in rodents from Asia and were
closely related to B. elizabethae, B. rattimassiliensis, and B.
tribocorum. The remaining genotypes belonged to B. henselae, B.
vinsonii, and B. tamiae. Among the positive febrile patients,
animal exposure was common: 36% reported owning either dogs or
cats and 71% reported rat exposure during the 2 weeks before
illness onset. The findings suggest that rodents are likely
reservoirs for a substantial portion of cases of human Bartonella
infections in Thailand.
