All Posts Tagged With: "RNA"

Full article: http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=20180313&retmode=ref&cmd=prlinks

Excerpt:

Ticks harbor numerous bacterial, protozoal, and viral pathogens
that can cause serious infections in humans and domestic animals.
Active surveillance of the tick vector can provide insight into
the frequency and distribution of important pathogens in the
environment. Nucleic-acid based detection of tick-borne
bacterial, protozoan, and viral pathogens requires the extraction
of both DNA and RNA (total nucleic acids) from ticks. Traditional
methods for nucleic acid extraction are limited to extraction of
either DNA or the RNA from a sample.
Here we present a simple bead-beating based protocol for
extraction of DNA and RNA from a single tick and show detection
of Borrelia burgdorferi and Powassan virus from individual,
infected Ixodes scapularis ticks. We determined expected yields
for total nucleic acids by this protocol for a variety of adult
tick species. The method is applicable to a variety of arthropod
vectors, including fleas and mosquitoes, and was partially
automated on a liquid handling robot.

Full article: http://www.medicalnewstoday.com/articles/196744.php

Excerpt:

A possible cause of irritable bowel syndrome has been traced to a small piece of RNA that blocks a substance protecting the colon membrane, leading to hostile conditions that can produce diarrhea, bloating and chronic abdominal pain.

New research shows that this RNA segment sends signals that stop the activity of the gene that produces glutamine, an amino acid. Previous research has linked a shortage of glutamine in the gut with the seepage of toxins and bacteria through the intestinal wall, irritating nerves and creating disease symptoms.

Scientists say that trying to generate glutamine in the disordered bowel by silencing this RNA segment could open up a whole new way of thinking about treating the diarrhea-predominant type of irritable bowel syndrome (IBS). In the meantime, they are making plans to conduct a clinical trial to see if glutamine supplements could also reduce common IBS symptoms

This form of the disorder is characterized by diarrhea and bloating as well as chronic abdominal pain that is difficult to treat. About a third of IBS patients have the diarrhea-predominant type, another third experience consistent constipation, and the rest experience alternating bouts of diarrhea and constipation.

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Linda

… from IMVA Medical News Commentaries list.

Yulia Tymoshenko, prime minister of Ukraine and a candidate in upcoming
national elections, visits a children’s hospital in western Ukraine.

Prime Minister Tymoshenko stated, “We cannot relax even for a moment because the World Health Organization (WHO) predicts two more waves of flu, including the bird flu, are expected in Ukraine. There is no alternative to vaccination. The entire world is going this way…” A day earlier she admitted she was not vaccinated and that she prefers “like all other people” plans to rely on garlic, onion and lemon as a way of preventing the flu. Commenting on what’s going on in Eastern Europe F. William Engdahl says, “The degree of fraud, deceit, official cover-up and outright criminal endangerment of the broad population by the current Swine Flu hysteria is seemingly without precedent.” Continued

J Clin Microbiol. 2009 Oct 21; [Epub ahead of print]

Delineation of a new species of the Borrelia burgdorferi sensu lato complex,
Borrelia americana sp.nov. Continued