All Posts Tagged With: "protozoal"

Extraction of total nucleic acids from ticks

Full article: http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=20180313&retmode=ref&cmd=prlinks

Excerpt:

Ticks harbor numerous bacterial, protozoal, and viral pathogens
that can cause serious infections in humans and domestic animals.
Active surveillance of the tick vector can provide insight into
the frequency and distribution of important pathogens in the
environment. Nucleic-acid based detection of tick-borne
bacterial, protozoan, and viral pathogens requires the extraction
of both DNA and RNA (total nucleic acids) from ticks. Traditional
methods for nucleic acid extraction are limited to extraction of
either DNA or the RNA from a sample.
Here we present a simple bead-beating based protocol for
extraction of DNA and RNA from a single tick and show detection
of Borrelia burgdorferi and Powassan virus from individual,
infected Ixodes scapularis ticks. We determined expected yields
for total nucleic acids by this protocol for a variety of adult
tick species. The method is applicable to a variety of arthropod
vectors, including fleas and mosquitoes, and was partially
automated on a liquid handling robot.

Lyme Disease: Arthritis by Infection

Linda’s Comment:  It amazes me that in the following publications do we find any suggestions about reducing our total body burden of pathogens and toxins.  It is a MUST that Lymies begin to reduce their total body burden of pathogens and toxins in order to begin addressing Lyme, Lyme Arthritis, Arthritis, and other chronic illness we see with Lyme patients.  Some people choose antibiotics…..I personally never went near antibiotics.  My whole detox and healing program was using anti-microbials, alternative medicine, alternative modalities, NO GMO foods, NO sugars, NO fast foods, NO soda’s, NO caffeine, NO coffee and I ate and still do eat organic foods.  I also have used a PhotonGenie since 2001 and use it daily.  I like it better than Rife, as I don’t have to worry about settings, I just turn it on and go.  I even sleep in mine.  The critters we Lymies fight LOVE heavy metals and especially GMO foods.  
 
There are also some foods that you don’t want to eat if you are having symptoms of Arthritis, however, it is more important to get rid of the GMO, sugars, coffee and soda’s to reduce the inflammation and pain.  The great thing about the fight program is you are dissolving biofilms and reducing inflammation on a daily basis.  So much of our pain comes from inflammation. 
 
I of course use many more things with my lifelong daily detox protocol.  If you can get IV chelation and do weekly colonics you can move things along faster.  However, you can start the program one step at a time and move at your own pace.  This is one protocol that must be done as suggested to get the full benefit of wellness.  The first three months are your toughest, but after that it is a breeze.  Yes, ever so often you have a day or two like you did when you first started, but I tell folks, it is like peeling an onion.  As you reach a new level you will have a couple of days where you keep your bathroom close.  At the end of 60 to 90 days and you begin to feel your life coming back to you, you will be very pleased that you began this journey.  Feel free to ask questions and I will share my journey with you.  Just remember JUST SAY NO TO GMO!! Continued

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