All Posts Tagged With: "immunohistochemistry"

The future of cancer diagnosis is changing rapidly. This new technique is very exciting and they mention that current techniques are woefully inadequate. This new approach is much faster and seems much more accurate. 

It does require taking a fine needle aspirate whereas cell search does not need biopsies but maybe the cell search technique will somehow become even better and could be synergistic with this new technique of protein analysis vs staining cells.

Although tumor cells obtained from human patients by image-guided intervention are a valuable source for diagnosing cancer, conventional means of analysis are limited. Here, we report the development of a Micro-NMR for Rapid Molecular Analysis of Human Tumor Samples
for rapid, multiplexed analysis of human tumors. We implemented the technology in a clinical setting to analyze cells obtained by fine-needle aspirates from suspected lesions in 50 patients and validated the results in an independent cohort of another 20 patients. Single fine-needle aspirates yielded sufficient numbers of cells to enable quantification of multiple protein markers in all patients within 60 min.

Full article: http://www.biomedcentral.com/content/pdf/1471-2202-11-121.pdf

Excerpt:

Conclusions

Anti-C. pneumoniae antibodies, obtained commercially, identified both typical intracellular and atypical extracellular C. pneumoniae antigens in frontal and temporal cortices of the AD brain. C. pneumoniae, amyloid deposits, and neurofibrillary tangles were present in the same regions of the brain in apposition to one another. Although additional studies are required to conclusively characterize the nature of Chlamydial immunoreactivity in the AD brain, these results further implicate C. pneumoniae infection with the pathogenesis of Alzheimer’s disease

Excerpt:

The significant association of plaque Lp-PLA2 with plaque macrophages and C. pneumoniae suggests an interactive role in accelerating inflammation in atherosclerosis. A possible mechanism for C. pneumoniaein the atherogenic process may involve infection of macrophages that induce Lp-PLA2 production leading to upregulation of inflammatory mediators in plaque tissue. Additional in vitro and in vivo research will be needed to advance our understanding of specific C. pneumoniae and Lp-PLA2 interactions in atherosclerosis.

Full article: http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pu
bmed&id=20332424&retmode=ref&cmd=prlinks

Excerpt:

Three pathologists graded immunoreactivity according
to a four-tier grading system: negative, weak, moderate, strong.
Canine parvoviral immunoreactivity was markedly decreased
following 2, 7, and 10 weeks of fixation in myocardium, small
intestine, and spleen, respectively. Bovine respiratory syncytial
virus immunoreactivity was markedly decreased following 7 weeks
of fixation. Bartonella henselae had an abrupt loss of
immunoreactivity following 9 weeks of fixation. Despite variation
among time points, immunoreactivity remained moderate to strong
throughout the study period for the other 18 antigens. These
results suggest that prolonged formalin fixation of up to 7 weeks
generally does not limit immunohistochemical detection of
infectious agents. However, the effects of prolonged fixation
depend on the targeted antigen and the selected antibody. The
results of this study further validate the utility and
reliability of immunohistochemistry in diagnostic pathology.

Background: Morphea, granuloma annulare (GA) and lichen sclerosus et atrophicans (LSA) have also been suggested to be linked to Borrelia infection. Previous studies based on serologic data or detection of Borrelia by immunohistochemistry and polymerase chain reaction (PCR) reported contradictory results. Thus, we examined skin biopsies of morphea, GA and LSA by PCR to assess the prevalence of Borrelia DNA in an endemic area and to compare our results with data in the literature.

Methods: Amplification of DNA sequences of Borrelia burgdorferi sensu lato by nested PCR from formalin-fixed and paraffin-embedded skin biopsies of morphea, GA and LSA, followed by automated sequencing of amplification products. PCR-based studies on Borrelia species in these disorders published until July 2009 were retrieved by a literature search. Continued